Associate Professor Shaun Lott
BSc (Hons), PhD
1994 University of Leeds, UK, PhD, Biochemistry and Molecular Biology
1990 University of Surrey, UK, BSc (1st Class Honours), Biochemistry
Research | Current
I am interested in using structural analysis and a range of biochemical and biophysical tools to address important biological systems in agriculture and human health. Current projects in the lab fall into three main groups:
Proteins of agricultural importance
These toxins are composed of three core subunits (A, B and C) and are best characterised from insect pathogens such as Yersinia entomophaga. Jason Busby has determined the first structure of the BC sub-complex, work that was recently published in Nature (Busby et al. 2013). Together with the structure of the A subunit previously determined by electron microscopy (Landsberg et al. 2011) and the structures of the associated chitinase enzymes (Busby et al. 2012), this gives us the first complete picture of this class of toxin. We are now working to produce engineered versions of the complex. This was is a collaboration with Mark Hurst at AgResearch and Michael Landsberg at the University of Queensland.
Non-ribosomal peptides synthases
Non-ribosomal peptide synthases (NRPSs) are large modular enzymes that produce an extensive range of secondary metabolites in bacteria and fungi. Verne Lee determined the structure of an adenylation domain from the endophytic fungus Neotyphodium lolii, the first fungal structure to be determined (Lee et al. 2010). He is now investigating the structure and function of the first archaeal NRPS enzyme to be identified.
Proteins from Mycobacterium tuberculosis known to be essential for pathogenesis
The tryptophan biosynthetic pathway is essential for M. tuberculosis to cause disease, and may also have a role in the interaction of the bacterium with the host immune system. We have determined the structures of the enzymes that catalyse the first two steps in tryptophan biosynthesis, and have developed inhibitors against them (Castell et al. 2013). This work has been carried out by Dr Genevieve Evans in collaboration with Prof Bill Denny.
M. tuberculosis scavenges iron from its host using the siderophore mycobactin. The first step in mycobactin synthesis is the production of salycilate. We have determined the structure of salycilate synthase (MbtI) and have developed some inhibitors of the enzyme is collaboration with Dr Richard Payne (Chi et al. 2012).
We are also working on the regulation of cholesterol metabolism by the transcriptional regulators KstR and KstR2. This work is an ongoing collaboration with Sharon Kendall.
Proteins that regulate the human epithelial sodium channel (ENaC) - the primary point of regulation for blood pressure
WW-containing E3 ubiquitin ligases
The cell surface expression of the epithelial sodium channel (ENaC) is regulated by ubiquitylation vis the ubiquitin ligases Nedd4 and Nedd4-2. We have investigated the interaction of the WW domains of Nedd4 with the target peptides in ENaC, using SPR (Lott et al. 2002) and NMR analysis (Bobby et al. 2013).
Teaching | Current
- Recipient of 2006 Queenstown Molecular Biology/Invitrogen Life Science Award
Areas of expertise
- Structural Biology
- Bacterial pathogenesis
Selected publications and creative works (Research Outputs)
- Gerth, M. L., Liu, Y., Jiao, W., Zhang, X.-X., Baker, E. N., Lott, J. S., ... Johnston, J. M. (2017). Crystal structure of a bicupin protein HutD involved in histidine utilization in Pseudomonas. Proteins, 85 (8), 1580-1588. 10.1002/prot.25303
Other University of Auckland co-authors: Jodie Johnston, Edward Baker
- Lott, J. S., & Lee, T. V. (2017). Revealing the Inter-Module Interactions of Multi-Modular Nonribosomal Peptide Synthetases. Structure (London, England : 1993), 25 (5), 693-695. 10.1016/j.str.2017.04.003
Other University of Auckland co-authors: Verne Lee
- Ho, N. A. T., Dawes, S. S., Crowe, A. M., Casabon, I., Gao, C., Kendall, S. L., ... Lott, J. S. (2016). The Structure of the Transcriptional Repressor KstR in Complex with CoA Thioester Cholesterol Metabolites Sheds Light on the Regulation of Cholesterol Catabolism in Mycobacterium tuberculosis. The Journal of biological chemistry, 291 (14), 7256-7266. 10.1074/jbc.m115.707760
Other University of Auckland co-authors: Stephanie Dawes, Edward Baker
- Busby, J. N., Lott, J. S., & Panjikar, S. (2016). Combining cross-crystal averaging and MRSAD to phase a 4354-amino-acid structure. Acta crystallographica. Section D, Structural biology, 72 (Pt 2), 182-191. 10.1107/s2059798315023566
Other University of Auckland co-authors: Jason Busby
- Lee, T. V., Johnson, R. D., Arcus, V. L., & Lott, J. S. (2015). Prediction of the substrate for nonribosomal peptide synthetase (NRPS) adenylation domains by virtual screening. Proteins, 83 (11), 2052-2066. 10.1002/prot.24922
Other University of Auckland co-authors: Verne Lee
- Bashiri, G., Johnston, J. M., Evans, G. L., Bulloch, E. M. M., Goldstone, D. C., Jirgis, E. N. M., ... Manos-Turvey, A. (2015). Structure and inhibition of subunit I of the anthranilate synthase complex of Mycobacterium tuberculosis and expression of the active complex. Acta crystallographica. Section D, Biological crystallography, 71 (Pt 11), 2297-2308. 10.1107/s1399004715017216
Other University of Auckland co-authors: Edward Baker, Ghader Bashiri, Jodie Johnston, Esther Bulloch, David Goldstone, Rochelle Ramsay
- Cookson, T. V. M., Evans, G. L., Castell, A., Baker, E. N., Lott, J. S., & Parker, E. J. (2015). Structures of Mycobacterium tuberculosis Anthranilate Phosphoribosyltransferase Variants Reveal the Conformational Changes That Facilitate Delivery of the Substrate to the Active Site. Biochemistry, 54 (39), 6082-6092. 10.1021/acs.biochem.5b00612
Other University of Auckland co-authors: Edward Baker
- Stubbing, L. A., Lott, J. S., Dawes, S. S., Furkert, D. P., & Brimble, M. A. (2015). Synthesis of DOHNAA, a Mycobacterium tuberculosis Cholesterol CD Ring Catabolite and FadD3 Substrate. European Journal of Organic Chemistry, 2015 (27), 6075-6083. 10.1002/ejoc.201500698
Other University of Auckland co-authors: Margaret Brimble, Daniel Furkert, Louise Stubbing, Stephanie Dawes