Dr Gus Grey
BTech(Hons, Biomedical Science) University of Auckland, PhD (Physiology) University of Auckland
I completed a Bachelor of Technology (Hons) in Biomedical Science at the University of Auckland, and continued on to complete a PhD in the Department of Physiology. During my doctoral studies I investigated the changing sub-cellular distribution patterns of membrane proteins in the ocular lens, and the functional consequences of these dynamic distributions.
Following my PhD, I took up a post-doctoral position at the Medical University of South Carolina with Prof Kevin Schey developing matrix-assisted laser desorption/ionisation (MALDI) imaging techniques to investigate the distribution of soluble and integral membrane proteins in the lens and other tissues, including brain, heart, lung and kidney.
I then moved to the mass spectrometry research centre at Vanderbilt University in Nashville, Tennessee to continue my post-doctoral studies using spatially-resolved proteomic techniques.
I am now a Senior Research Fellow in the Department of Physiology, direct the Biomedical Imaging Research Unit, and coordinate the University of Auckland Mass Spectrometry Hub.
Research | Current
I am interested in applying spatially resolved mass spectrometric techniques, such as MALDI imaging, to investigate the molecular basis of ocular diseases. My current research aims to understand the changes that take place to lens metabolites and small molecules in specific regions of the lens in aging and cataract formation. Specifically, I am interested in monitoring the metabolic pathways responsible for maintaining glutathione (GSH) in the nucleus of the ocular lens with the long term view of developing effective therapeutic strategies to prevent age-related nuclear (ARN) cataract. The interaction of lens small molecules with lens proteins, and the implications for these interactions in lens pathologies is also a focus of my research.
Additionally, I collaborate with the Centre for Brain Research to study changes to the spatial distributions and concentrations of biomolecules in specific brain regions affected by major neurodegenerative diseases such as Alzheimer's and Huntington's disease.
Teaching | Current
MEDSCI 733 - Advanced Methods in Cell Physiology (Light Microscopy Module)
PHARMACY 751 - Pharmaceutical Techniques
Sir Charles Hercus Health Research Fellow
Director, Biomedical Imaging Research Unit
Coordinator, University of Auckland Mass Spectrometry Hub
Selected publications and creative works (Research Outputs)
- Hunter, M., Demarais, N. J., Faull, R. L. M., Grey, A. C., & Curtis, M. A. (2018). Layer-specific lipid signatures in the human subventricular zone demonstrated by imaging mass spectrometry. Scientific reports, 8 (1)10.1038/s41598-018-20793-4
Other University of Auckland co-authors: Richard Faull, Maurice Curtis, Nicholas Demarais
- Karunasinghe, R., Grey, A., Telang, R., Vlajkovic, S., & Lipski, J. (2017). Differential spread of anoxic depolarization contributes to the pattern of neuronal injury after oxygen and glucose deprivation (OGD) in the Substantia Nigra in rat brain slices. Neuroscience, 340, 359-372. 10.1016/j.neuroscience.2016.10.067
Other University of Auckland co-authors: Janusz Lipski, Ravi Telang, Srdjan Vlajkovic, Rashika Karunasinghe
- Grey, A., Demarais, N., West, B., & Donaldson, P. (2017). A quantitative map of glutathione in the aging human lens. International Journal of Mass Spectrometry10.1016/j.ijms.2017.10.008
Other University of Auckland co-authors: Nicholas Demarais, Paul Donaldson
- Nye-Wood, M. G., Spraggins, J. M., Caprioli, R. M., Schey, K. L., Donaldson, P. J., & Grey, A. C. (2017). Spatial distributions of glutathione and its endogenous conjugates in normal bovine lens and a model of lens aging. Experimental Eye Research, 154, 70-78. 10.1016/j.exer.2016.11.008
Other University of Auckland co-authors: Mitchell Nye-Wood, Paul Donaldson
- Schey, K. L., Hachey, A. J., Rose, K. L., & Grey, A. C. (2016). MALDI imaging mass spectrometry of Pacific White Shrimp L. vannamei and identification of abdominal muscle proteins. Proteomics, 16 (11-12), 1767-1774. 10.1002/pmic.201500531
- Grey, A. C. (2016). MALDI imaging of the eye: Mapping lipid, protein and metabolite distributions in aging and ocular disease. International Journal of Mass Spectrometry, 401, 31-38. 10.1016/j.ijms.2016.02.017
- Petrova, R. S., Schey, K. L., Donaldson, P. J., & Grey, A. C. (2015). Spatial distributions of AQP5 and AQP0 in embryonic and postnatal mouse lens development. Experimental Eye Research, 132, 124-135. 10.1016/j.exer.2015.01.011
Other University of Auckland co-authors: Paul Donaldson, Rosica Petrova
- Schey, K. L., Grey, A. C., & Nicklay, J. J. (2013). Mass spectrometry of membrane proteins: a focus on aquaporins. Biochemistry, 52 (22), 3807-3817. 10.1021/bi301604j