Dr Gus Grey

BTech(Hons), PhD

Biography

Gus completed a Bachelor of Technology (Hons) in Biomedical Science at The University of Auckland, and continued on to complete a PhD in the Department of Physiology. During his doctoral studies he investigated the changing sub-cellular distribution patterns of membrane proteins in the ocular lens, and the functional consequences of these dynamic distributions.

Following his PhD, Gus took up a post-doctoral position at the Medical University of South Carolina with Prof Kevin Schey developing matrix-assisted laser desorption/ionisation (MALDI) imaging techniques to investigate the distribution of soluble and integral membrane proteins in the lens and other tissues, including brain, heart, lung and kidney.

He then moved to the mass spectrometry research centre at Vanderbilt University in Nashville, Tennessee to continue his post-doctoral studies using spatially-resolved proteomic techniques.

Gus is now a senior research fellow in the Molecular Vision Laboratory in the Department of Physiology and is funded by a Sir Charles Hercus Health Research fellowship.

Research | Current

Gus is interested in applying spatially resolved mass spectrometric techniques, such as MALDI imaging, to investigate the molecular basis of ocular diseases. His current research aims to understand how long-lived lens proteins are modified over time to contribute to normal lens function, and which protein modifications lead to lens cataract. Additionally, Gus is using MALDI imaging to map the distributions of small molecules and metabolites to distinct regions of the lens, and to monitor the metabolic pathways responsible for maintaining glutathione (GSH) in the nucleus of the ocular lens with the long term view of developing effective therapeutic strategies to prevent age-related nuclear (ARN) cataract.

Primary research skills include MALDI Mass Spectrometry, Imaging Mass Spectrometry, Cryosectioning, 1D SDS-PAGE/Western Blot, Immunohistochemistry and Confocal Microscopy.

Distinctions/Honours

Sir Charles Hercus Health Research Fellow

Selected publications and creative works (Research Outputs)

  • Petrova, R. S., Schey, K. L., Donaldson, P. J., & Grey, A. C. (2015). Spatial distributions of AQP5 and AQP0 in embryonic and postnatal mouse lens development. Experimental eye research, 132, 124-135. 10.1016/j.exer.2015.01.011
    Other University of Auckland co-authors: Paul Donaldson, Rosica Petrova
  • Chang, L. Y. L., Lowe, J., Ardiles, A., Lim, J., Grey, A. C., Robertson, K., ... Acosta, M. L. (2014). Alzheimer's disease in the human eye. Clinical tests that identify ocular and visual information processing deficit as biomarkers. Alzheimers Dement, 10 (2), 251-261. 10.1016/j.jalz.2013.06.004
    Other University of Auckland co-authors: Monica Acosta, Julie Lim, Lily Chang
  • Schey, K. L., Grey, A. C., & Nicklay, J. J. (2013). Mass spectrometry of membrane proteins: a focus on aquaporins. Biochemistry, 52 (22), 3807-3817. 10.1021/bi301604j
    URL: http://hdl.handle.net/2292/23821
  • Grey, A. C., Walker, K. L., Petrova, R. S., Han, J., Wilmarth, P. A., David, L. L., ... Schey, K. L. (2013). Verification and spatial localization of aquaporin-5 in the ocular lens. Exp Eye Res, 108, 94-102. 10.1016/j.exer.2012.12.004
    Other University of Auckland co-authors: Paul Donaldson, Rosica Petrova
  • Grey, A. C., & Schey, K. L. (2009). Age-related changes in the spatial distribution of human lens alpha-crystallin products by MALDI imaging mass spectrometry. Investigative Ophthalmology & Visual Science, 50 (9), 4319-4329. 10.1167/iovs.09-3522
    URL: http://hdl.handle.net/2292/17585
  • Grey, A. C., Chaurand, P., Caprioli, R. M., & Schey, K. L. (2009). MALDI imaging mass spectrometry of integral membrane proteins from ocular lens and retinal tissue. Journal of Proteome Research, 8 (7), 3278-3283. 10.1021/pr800956y
    URL: http://hdl.handle.net/2292/17584
  • Grey, A. C., Li, L., Jacobs, M. D., Schey, K. L., & Donaldson, P. J. (2009). Differentiation-dependent modification and subcellular distribution of aquaporin-0 suggests multiple functional roles in the rat lens. Differentiation, 77 (1), 70-83. 10.1016/j.diff.2008.09.003
    URL: http://hdl.handle.net/2292/7645
    Other University of Auckland co-authors: Paul Donaldson

Identifiers

Contact details

Primary location

M&HS BUILDING 502 - Bldg 502
Level 4, Room 420
85 PARK RD
GRAFTON
AUCKLAND 1023
New Zealand

Web links