Mr Akarsh Mathrani

BSc (Biological Sciences); PG Dip (Genetics); MSc (Genetics) with Distinction

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Doctoral Candidate - Doctor of Philosophy

Biography

During my MSc program, I investigated the DNA replication and chromosome segregation dynamics of Pseudomonas fluorescens SBW25, a gram-negative, plant-associated bacterium. The study involved incorporating arrays of repeated operator regions bound by their fluorescently-labelled cognate repressors. Origin-proximal arrays could therefore be visualised as fluorescent foci upon live-cell imaging and analysed to follow the chromosome replication and segregation process in single living cells. The study shows evidence for concurrent DNA replication cycles occurring in P. fluorescens SBW25. This process appears to be exacerbated in nutrient-rich media (LB) as opposed to cells grown in nutrient-poor media (M9-glycerol). Moreover, asynchronous DNA replication initiations were also observed, and appear to be a common phenomenon when cells are grown in nutrient-rich media but not in nutrient-poor media. The study sheds light on a key cellular process in the Pseudomonads, a genus where DNA replication has not been studied extensively. The whole thesis can be found at: http://mro.massey.ac.nz/handle/10179/7328

Research | Current

Presently, as a doctoral candidate, I am working on characterising the association between type 2 diabetes mellitus and the human gut microbiota.

Supervisors:

A. Prof. Mike Taylor

Prof. Sally Poppit

Prof. Garth Cooper

Dr. Jackie Aitken

Distinctions/Honours

Achieved a Distinction in Master of Science (Genetics) from Massey University

Areas of expertise

Microbiology and Genetics

Contact details

Primary office location

THOMAS BUILDING EXTENSION - Bldg 110N
Level 3, Room 3001
3A SYMONDS ST
AUCKLAND CENTRAL
AUCKLAND 1010
New Zealand