Mr Brad Joseph Raos

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Doctoral Candidate - Doctor of Philosophy

Research | Current

Thesis

Controlled three dimensional growth of human brain cells in hydrogels

In many neurological conditions, such as epilepsy, a break down of normal cellular communication is observed. Historically, insight into these conditions has been gained by growing brain cells in vitro, i.e. outside the body. However, while cell signaling in two-dimensional in vitro cultures is well understood little has been done in three-dimensional cultures. In order to fully understand cellular processes it is necessary to replicate as closely as possible the conditions in living tissues. For realistic brain models it is important to investigate the impact that topology has on cell signaling in three-dimensional geometries to determine how this extra degree of freedom alters signaling behaviour of the network. The insight gleaned from this information may help in the development of neurological conditions that results from a malfunction in cellular communication.

This project aims to control the growth of human neurons, derived from a cell line, in 3D hydrogels. Once grown in a defined geometry the neuronal culture will be coupled with our existing multielectrode array technology and calcium fluorescence techniques to investigate the behaviour of the cells.
 

Supervisors

Research areas

  • Neuroengineering
  • Neuroscience
     

Research groups

Signal processing

 

Selected publications

Raos, B. J., C. P. Unsworth, J. L. Costa, C. A. Rohde, C. S. Doyle, A. S. Bunting, E. Delivopoulos, et al. Infra-Red Laser Ablative Micromachining of Parylene-C on SiO2 Substrates for Rapid Prototyping, High Yield, Human Neuronal Cell Patterning. Biofabrication 5, no. 2 (Jun 2013): 025006.

Selected publications and creative works (Research Outputs)

  • Raos, B. J., Graham, E. S., & Unsworth, C. P. (2017). Nanosecond UV lasers stimulate transient Ca2+ elevations in human hNT astrocytes. Journal of Neural Engineering, 14 (3)10.1088/1741-2552/aa5f27
    Other University of Auckland co-authors: Charles Unsworth, Scott Graham
  • Jordan, M. D., Raos, B. J., Bunting, A. S., Murray, A. F., Graham, E. S., & Unsworth, C. P. (2016). Human astrocytic grid networks patterned in parylene-C inlayed SiO2 trenches. Biomaterials, 105, 117-126. 10.1016/j.biomaterials.2016.08.006
    URL: http://hdl.handle.net/2292/30620
    Other University of Auckland co-authors: Charles Unsworth, Scott Graham
  • Raos, B. J., Graham, E. S., Murray, A. F., Simpson, M. C., & Unsworth, C. P. (2016). Investigating Parylene-HT as a Substrate for Human Cell Patterning. Paper presented at 38th Annual International Conference of the IEEE, Engineering in Medicine and Biology Society (EMBC 2016), Orlando, Florida, US. 16 August - 20 August 2016. 2016 IEEE 38th Annual International Conference of the Engineering in Medicine and Biology Society (EMBC). (pp. 4). 10.1109/EMBC.2016.7590660
    Other University of Auckland co-authors: Charles Unsworth, Scott Graham, Cather Simpson
  • Raos, B. J., Graham, E. S., & Unsworth, C. P. (2016). Investigation of the Ca2+ Response of human hNT astrocytes to laser removal of cellular processes. 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), 1750-1753. Orlando, FL, USA: Institute of Electrical and Electronics Engineers. 10.1109/EMBC.2016.7591055
    Other University of Auckland co-authors: Charles Unsworth, Scott Graham
  • Raos, B. J., Unsworth, C. P., Costa, J. L., Rohde, C. A., Doyle, C. S., Delivopoulos, E., ... Graham, E. S. (2013). Low cost, patterning of human hNT brain cells on parylene-C with UV& IR laser machining.. Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference.
    Other University of Auckland co-authors: Charles Unsworth, Michelle Dickinson, Scott Graham
  • Raos, B. J., Unsworth, C. P., Costa, J. L., Rohde, C. A., Doyle, C. S., Bunting, A. S., ... Simpson, M. C. (2013). Infra-red laser ablative micromachining of parylene-C on SiO2 substrates for rapid prototyping, high yield, human neuronal cell patterning. Biofabrication, 5 (2)10.1088/1758-5082/5/2/025006
    Other University of Auckland co-authors: Michelle Dickinson, Charles Unsworth, Scott Graham, Cather Simpson
  • Raos, B. J., Unsworth, C. P., Costa, J. L., Rohde, C. A., Doyle, C. S., Delivopoulos, E., ... Graham, E. S. (2013). Low cost, patterning of human hNT brain cells on parylene-C with UV& IR laser machining.. United States. Conf Proc IEEE Eng Med Biol Soc. 10.1109/EMBC.2013.6609637
    Other University of Auckland co-authors: Michelle Dickinson, Scott Graham, Cather Simpson

Contact details

Primary location

UNISERVICES HOUSE - Bldg 439
Level 3, Room 325
70 SYMONDS ST
AUCKLAND 1010
New Zealand