Dr David Charles Goldstone
David Goldstone is a Rutherford Discovery Fellow and Senior Lecturer in Structural Biology. He has been a member of the Faculty since 2012. He obtained his PhD form the University of Auckland and was a post-doctoral fellow with the AgResearch Structural Biology Lab and MRC National Institute for Medical Research in London, UK before returning to NZ.
Research | Current
Structure and Function of Anti-Retroviral Host Proteins
I am interested in how HIV and other retroviruses interact with components of the host cell during infection. These interactions govern both the ability of the virus to reproduce, and the ability of the cell to block and contain infection. Of particular interest are members of the TRIM protein family that can recognise retroviral components within the cell and then act to block the viral lifecycle, disrupting infection. We aim to characterise these proteins and the interactions they make using X-ray crystallography, small-angle X-ray scattering and other biophysical techniques.
How to metal based chemotherapeutics target proteins?
Platinum compounds form the foundation of many treatment regimens for cancer. However, these compounds are promiscious lacking the specificity of other chemotherapeutic compounds. This results in off target and side-effects that limit their usability in treatment. We are interested in how metal-based compounds interact with proteins, and understanding how we can generate specificity to improve treatment options for cancer patients.
Teaching | Current
BIOSCI201 - Course director - Module B: The central dogma of molecular biology
BIOSCI203 - Module C: Protein assembly and molecular machines
BIOSCI759 - HIV evasion of cellular defense to infection
BIOSCI746 - Protein assembly and its investgation by AUC and light-scattering
Rutherford Discovery Fellow
Areas of expertise
Structural Biology, Protein-protein interactions, Protein ligand interactions, Small Angle X-ray scattering
Chair - SBS Research Committee
Selected publications and creative works (Research Outputs)
- Acton, O., Grant, T., Nicastro, G., Ball, N. J., Goldstone, D. C., Robertson, L. E., ... Stoye, J. P. (2019). Structural basis for Fullerene geometry in a human endogenous retrovirus capsid. Nature communications, 10 (1)10.1038/s41467-019-13786-y
- Sun, Y., Keown, J. R., Black, M. M., Raclot, C., Demarais, N., Trono, D., ... Goldstone, D. C. (2019). A Dissection of Oligomerization by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family. Journal of molecular biology, 431 (14), 2511-2527. 10.1016/j.jmb.2019.05.002
Other University of Auckland co-authors: Nicholas Demarais
- Keown, J. R., Black, M. M., Ferron, A., Yap, M., Barnett, M. J., Pearce, F. G., ... Goldstone, D. C. (2018). A helical LC3-interacting region mediates the interaction between the retroviral restriction factor Trim5α and mammalian autophagy-related ATG8 proteins. The Journal of biological chemistry, 293 (47), 18378-18386. 10.1074/jbc.ra118.004202
- Sullivan, M. P., Nieuwoudt, M. K., Bowmaker, G. A., Lam, N. Y. S., Truong, D., Goldstone, D. C., & Hartinger, C. G. (2018). Unexpected arene ligand exchange results in the oxidation of an organoruthenium anticancer agent: the first X-ray structure of a protein-Ru(carbene) adduct. Chemical communications (Cambridge, England), 54 (48), 6120-6123. 10.1039/c8cc02433b
Other University of Auckland co-authors: Christian Hartinger, Matthew Sullivan, Dianna Truong, Michel Nieuwoudt, Graham Bowmaker, Cather Simpson
- Pinheiro, J., Biboy, J., Vollmer, W., Hirt, R., Keown, J., Artuyants, A., ... Simoes-Barbosa, A. (2018). The protozoan Trichomonas vaginalis targets bacteria with laterally-acquired NlpC/P60 peptidoglycan hydrolases. 10.1101/320382
Other University of Auckland co-authors: Augusto Barbosa
- Goldstone, D. C., Walker, P. A., Calder, L. J., Coombs, P. J., Kirkpatrick, J., Ball, N. J., ... Stoye, J. P. (2014). Structural studies of postentry restriction factors reveal antiparallel dimers that enable avid binding to the HIV-1 capsid lattice. Proceedings of the National Academy of Sciences of the United States of America, 111 (26), 9609-9614. 10.1073/pnas.1402448111
- Goldstone, D. C., Flower, T. G., Ball, N. J., Sanz-Ramos, M., Yap, M. W., Ogrodowicz, R. W., ... Stoye, J. P. (2013). A unique spumavirus Gag N-terminal domain with functional properties of orthoretroviral matrix and capsid. PLoS Pathog, 9 (5)10.1371/journal.ppat.1003376
- Goldstone, D. C., Ennis-Adeniran, V., Hedden, J. J., Groom, H. C. T., Rice, G. I., Christodoulou, E., ... Yap, M. W. (2011). HIV-1 restriction factor SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase. NATURE, 480 (7377), 379-383. 10.1038/nature10623
Primary office location
THOMAS BUILDING EXTENSION - Bldg 110N
Level 4, Room 4004
3A SYMONDS ST