Mr James Hucklesby
Research | Current
I completed my Bachelor of Science and Bachelor of Biomedical Science (Honours) at the University of Auckland, and am now working towards my PhD in Biological Sciences, supervised by Scott Graham and Kate Angel.
My research focuses on the endothelium, the innermost layer of cells lining the blood vessels of the body. In particular, I am interested in how these cells interact with the plasmin system, which is responsible for regulating blood clotting. This research has involved using microfluidic technology to study how T cells interact with the endothelium under shear stress conditions that mimic blood vessels and using Electrical Cell-Substrate Impedance Sensing to measure how endothelial cells respond to stroke-like conditions in real-time.
I have also presented in a variety of settings, serving as the president of Varsity Toastmasters and helping to organise orientation programs for new students.
Second Place - University of Auckland Exposure Competition (2018)
Runner Up - University of Auckland 3 Minute Thesis Competition (2019)
Best in group - University of Auckland Biomedical Science Honours Symposium (2017)
Third Place - Toastmasters New Zealand TableTopics Competition (2017)
Areas of expertise
Electrical Cell-Substrate Impedance Sensing (ECIS), plasmin system proteins, immune cell interactions under shear stress, endothelial cell culture.
Selected publications and creative works (Research Outputs)
- Anchan, A., Kalogirou-Baldwin, P., Johnson, R., Kho, D. T., Joseph, W., Hucklesby, J., ... Graham, E. S. (2019). Real-Time Measurement of Melanoma Cell-Mediated Human Brain Endothelial Barrier Disruption Using Electric Cell-Substrate Impedance Sensing Technology. Biosensors, 9 (2).10.3390/bios9020056
Other University of Auckland co-authors: Rebecca Johnson, Akshata Anchan, Wayne Joseph, Graeme Finlay, Simon OCarroll, Scott Graham, Catherine Angel