Dr Mia Jullig
Research | Current
My primary interest is in applied proteomics using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS. This combination enables identification and comparison of the relative levels of hundreds of proteins in complex samples simultaneously, with 8 different samples being compared side-by-side within one LC-MS/MS run. I use the iTRAQ approach to investigate proteomic changes in key tissues in various disease states, e.g. cardiac, vascular and renal proteomes in diabetes and hypertension. Because reduction in sample complexity equals increased resolution, I often focus on mitochondria isolated from tissues of interest.
Due to my longstanding fascination with mitochondria, I in 2009 took the initiative to start The Mitochondrial Network of New Zealand. This is a nationwide society aimed at facilitating the sharing of knowledge and ideas between all researchers working with mitochondria in New Zealand.
A key aspect of my proteomic work is the manual post-processing of the LC-MS/MS output data. I am particularly interested in optimizing the accuracy of the iTRAQ technique by in silico correction for virtually unavoidable sources of error, e.g. unequal loading and uneven purity of samples processed to reduce complexity.
Small differences in protein abundance can make a big difference biologically, especially if a whole pathway is altered in the same direction. In addition to determining changes at the level of the individual proteins, I therefore also analyse how functional groups of proteins, e.g. defined pathways and subunits in protein complexes, behave as a group.
Another critical point is data presentation and final interpretation of the results in their biological context. Proteomics is sometimes accused of merely generating “endless lists of proteins” which can be hard to interpret and are rarely very inspiring. I therefore aim to organize the data not only in functional groups but to create comprehensive illustrations to make the results accessible at a glance.
Selected publications and creative works (Research Outputs)
- Crossman, D. J., Shen, X., Jüllig M, Munro, M., Hou, Y., Middleditch, M., ... Dos Remedios, C. G. (2017). Increased collagen within the transverse tubules in human heart failure. Cardiovascular research, 113 (8), 879-891. 10.1093/cvr/cvx055
Other University of Auckland co-authors: David Crossman, Christian Soeller, David Baddeley, Martin Middleditch
- Koschwanez, H., Robinson, H., Beban, G., MacCormick, A., Hill, A., Windsor, J., ... Broadbent, E. (2017). Randomized clinical trial of expressive writing on wound healing following bariatric surgery. Health Psychology, 36 (7), 630-640. 10.1037/hea0000494
Other University of Auckland co-authors: John Windsor, Elizabeth Broadbent, Andrew MacCormick, Andrew Hill, Roger Booth
- Murphy, R., Tsai, P., Jüllig M, Liu, A., Plank, L., & Booth, M. (2017). Differential Changes in Gut Microbiota After Gastric Bypass and Sleeve Gastrectomy Bariatric Surgery Vary According to Diabetes Remission. Obesity surgery, 27 (4), 917-925. 10.1007/s11695-016-2399-2
Other University of Auckland co-authors: Lindsay Plank, Rinki Murphy, Peter Tsai, Amy Liu
- Biswas, K., Wagner Mackenzie, B., Waldvogel-Thurlow, S., Middleditch, M., Jullig, M., Zoing, M., ... Douglas, R. G. (2017). Differentially Regulated Host Proteins Associated with Chronic Rhinosinusitis Are Correlated with the Sinonasal Microbiome. Frontiers in cellular and infection microbiology, 710.3389/fcimb.2017.00504
Other University of Auckland co-authors: Kristi Biswas, Richard Douglas, Mike Taylor, Brett Wagner
- Koschwanez, H., Robinson, H., Beban, G., MacCormick, A., Hill, A., Windsor, J., ... Broadbent, E. (2016). A RANDOMIZED CONTROLLED TRIAL INVESTIGATING THE EFFECTS OF AN EXPRESSIVE WRITING INTERVENTION ON SURGICAL WOUND HEALING IN BARIATRIC PATIENTS. INTERNATIONAL JOURNAL OF BEHAVIORAL MEDICINE. (pp. 1).
Other University of Auckland co-authors: John Windsor, Elizabeth Broadbent
- Xu, J., Begley, P., Church, S. J., Patassini, S., Hollywood, K. A., Jüllig M, ... Unwin, R. D. (2016). Graded perturbations of metabolism in multiple regions of human brain in Alzheimer's disease: Snapshot of a pervasive metabolic disorder. Biochimica et biophysica acta, 1862 (6), 1084-1092. 10.1016/j.bbadis.2016.03.001
Other University of Auckland co-authors: Garth Cooper, Maurice Curtis, Richard Faull, Henry Waldvogel
- Xu, J., Jüllig M, Middleditch, M. J., & Cooper, G. J. S. (2015). Modelling atherosclerosis by proteomics: Molecular changes in the ascending aortas of cholesterol-fed rabbits. Atherosclerosis, 242 (1), 268-276. 10.1016/j.atherosclerosis.2015.07.001
Other University of Auckland co-authors: Garth Cooper, Martin Middleditch
- Jullig, M., Middleditch, M., & Cooper, G. (2014). Lessons learned during eight years of using iTRAQ in the proteomic study of pathology. Paper presented at Eleventh International Symposium on Mass Spectrometry in the Health& Life Sciences, San Francisco, USA. 17 August - 21 August 2014. Molecular & Cellular Proteomics.