Mrs Lola Mugisho

Master of Science (Hons) in Biomedical Science

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Doctoral Candidate - Doctor of Philosophy

Research | Current

My current research investigates the role of gap junction proteins (connexins) in diabetic retinopathy with a primary focus on vascular disruptions seen in the disease. My research aims to:

  • Develop a diabetic retinopathy model that characterises connexin43 changes seen in human tissues,
  • Describe the role of connexins and its contribution to the pathology of diabetic retinopathy,
  • Evaluate possible connexin-based therapies for the treatment of diabetic retinopathy.

Distinctions/Honours

International Gap Junction Conference (IGJC) Travel Award (2017)

Buchanan Ocular Therapeutics Unit Doctoral Scholarship (2015)

Faculty of Science Masters Awards (2013)

FMHS Summer Studentship Scholarship (2012/2013)

 

Areas of expertise

  • Animal handling
  • in vivo rodent retinal imaging techniques (Fundus Imaging and Spectral Domain-Optical Coherence Tomography (OCT)
  • Cell culture
  • Western blotting
  • Flow cytometry
  • Cytometric Bead Array (CBA)
  • Spectrophotometry
  • Confocal cell and tissue imaging
  • Tissue sectioning using a cryostat
  • Immunocytochemistry
  • Immunohistochemistry

 

Selected publications and creative works (Research Outputs)

  • Mugisho, O. O., Green, C. R., Zhang, J., Binz, N., Acosta, M. L., Rakoczy, E., & Rupenthal, I. D. (2017). Immunohistochemical Characterization of Connexin43 Expression in a Mouse Model of Diabetic Retinopathy and in Human Donor Retinas. Int J Mol Sci, 18 (12).10.3390/ijms18122567
    Other University of Auckland co-authors: Colin Green, Jie Zhang, Monica Acosta, Ilva Rupenthal
  • Mugisho, O. O., Green, C. R., Kho, D. T., Zhang, J., Scott Graham, E., Acosta, M. L., & Rupenthal, I. D. (2017). The inflammasome pathway is amplified and perpetuated in an autocrine manner through connexin43 hemichannel mediated ATP release. Biochimica et biophysica acta10.1016/j.bbagen.2017.11.015
    Other University of Auckland co-authors: Ilva Rupenthal, Colin Green, Monica Acosta, Scott Graham, Jie Zhang
  • Rotimi, O. O., Green, C., Zhang, J., Acosta, M. L., & Rupenthal, I. (2016). Increased Connexin43 expression in a mouse model of advanced diabetic retinopathy. Paper presented at Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Seattle, WA. 1 May - 5 May 2016. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. (pp. 2).
    Other University of Auckland co-authors: Colin Green, Ilva Rupenthal, Monica Acosta
  • Choi, J. M., Rotimi, O. O., O'Carroll SJ, & Nicholson, L. F. B. (2016). IL-6 stimulates a concentration-dependent increase in MCP-1 in immortalised human brain endothelial cells. F1000Research, 510.12688/f1000research.8153.2
    Other University of Auckland co-authors: Simon OCarroll
  • O'Carroll SJ, Kho, D. T., Wiltshire, R., Nelson, V., Rotimi, O., Johnson, R., ... Graham, E. S. (2015). Pro-inflammatory TNFα and IL-1β differentially regulate the inflammatory phenotype of brain microvascular endothelial cells. Journal of neuroinflammation, 1210.1186/s12974-015-0346-0
    URL: http://hdl.handle.net/2292/30036
    Other University of Auckland co-authors: Scott Graham, Simon OCarroll, Catherine Angel, Dan Kho
  • Rotimi, O. O. (2013). Influence of Downstream Peripheral Inflammation Markers on an in vitro Model of the Blood-Brain Barrier The University of Auckland. ResearchSpace@Auckland.
    URL: http://hdl.handle.net/2292/22551