Dr Peter Stuart Freestone

BSc (Hons) Biomedical Science, PhD Physiology

Research | Current

Peter is a recent graduate of the University of Auckland, completing his PhD studies under the supervision of Prof Janusz Lipski 'Effects of rotenone and 6-OHDA on dopaminergic neurons of the substantia nigra studied in vitro'. Since returning from a post-doctotal position in Rome Italy, Peter has established an independent line of research studying normal brain function and connectivity between brain cells.

The brain produces its own cannabis-like substances and these compounds - called endocannabinoids - are involved in many functions from regulating immune responses to modulating the flow of information between brain cells. They also show promise as a treatment (on their own, or in conjunction with other strategies) for various neurological disorders such as Multiple Sclerosis and Parkinson's disease (PD)

Peter's current research interest is probing the neuro-modulatory role of endocannabinoids in the basal ganglia - an important brain network associated with movement, reward and learning. This network connects several brain structures and the loss of dopamine producing cells in one of these structures (the Substantia Nigra pars compacta) underlies PD.

Recording of electrical activity from single rodent brain cells (in vitro) enables Peter to measure changes in the strength of connections between neurons, and also the modulation of connections by endocannabinoids.

More recently, Peter has established advanced optogenetic techniques at the University. These techniques enable light to very selectively activate particular neurons which have been made to possess light-activated proteins such as channelrhodopsin. This important technique holds great potential for neuroscience research enabling very precise dissection of neural networks not previously possible with conventional electrical stimulation approaches. In addition, future therapeutic strategies for diseases ranging from PD and epilepsy, to cardiac arrhythmias may be treated with optogenetic tools.

Collaborations:

  • Prof Janusz Lipski, Department of Physiology and Centre for Brain Research, UoA

  • Prof George Augustine, Nangyang Technological University, Singapore

  • Prof Simon Malpas and Dr David Budgett, Auckland Bioengineering Institute, UoA

  • Prof Nicola Mercuri, Fondazione Santa Lucia, Rome Italy

  • A/Prof Bronwen Conner, Centre for Brain Research, UoA

 

Teaching | Current

MedSci 309: Biophysics of Nerve and Muscle

MedSci 317: Integrative Neuroscience: From Fetus to Adult

MedSci 733: Advanced Methods in Cell Physiology

Areas of expertise

  • Electrophysiology (whole-cell patch-clamp, single unit extracellular)

  • Acute brain slice preparation

  • Intracerebral viral vector injections

  • Optogenetic stimulation (functional mapping etc.)

  • Immunocytochemistry

Selected publications and creative works (Research Outputs)

  • Kim, Y., Griffin, J. M., Nor, M. N. M., Zhang, J., Freestone, P. S., Danesh-Meyer, H. V., ... O'Carroll SJ (2017). Tonabersat Prevents Inflammatory Damage in the Central Nervous System by Blocking Connexin43 Hemichannels. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics10.1007/s13311-017-0536-9
    Other University of Auckland co-authors: Simon OCarroll, Jarred Griffin, Helen Danesh-Meyer, Ilva Rupenthal, Monica Acosta, Colin Green, Mohd Nasir Mat Nor, Jie Zhang
  • Chen, F.-Y. B., Budgett, D. M., Sun, Y., Malpas, S., McCormick, D., & Freestone, P. S. (2017). Pulse-Width Modulation of Optogenetic Photo-Stimulation Intensity for Application to Full-Implantable Light Sources. IEEE Transactions on Biomedical Circuits and Systems, 11 (1), 28-34. 10.1109/TBCAS.2016.2577042
    URL: http://hdl.handle.net/2292/32121
    Other University of Auckland co-authors: David Budgett, Yuhui Sun, Daniel McCormick, Beverly Chen, Simon Malpas
  • Freestone, P. S., Wu, X. H., de Guzman, G., & Lipski, J. (2015). Excitatory drive from the Subthalamic nucleus attenuates GABAergic transmission in the Substantia Nigra pars compacta via endocannabinoids. European journal of pharmacology, 767, 144-151. 10.1016/j.ejphar.2015.09.050
    URL: http://hdl.handle.net/2292/33071
    Other University of Auckland co-authors: Janusz Lipski
  • Yee, A. G., Lee, S.-M., Hunter, M. R., Glass, M., Freestone, P. S., & Lipski, J. (2014). Effects of the Parkinsonian toxin MPP+ on electrophysiological properties of nigral dopaminergic neurons. Neurotoxicology, 45, 1-11. 10.1016/j.neuro.2014.08.009
    Other University of Auckland co-authors: Michelle Glass, Janusz Lipski
  • Freestone, P. S., Guatteo, E., Piscitelli, F., di Marzo, V., Lipski, J., & Mercuri, N. B. (2014). Glutamate spillover drives endocannabinoid production and inhibits GABAergic transmission in the Substantia Nigra pars compacta. Neuropharmacology, 79, 467-475. 10.1016/j.neuropharm.2013.12.007
    Other University of Auckland co-authors: Janusz Lipski
  • Chung, K. K. H., Freestone, P. S., & Lipski, J. (2011). Expression and functional properties of TRPM2 channels in dopaminergic neurons of the substantia nigra of the rat. J Neurophysiol, 106 (6), 2865-2875. 10.1152/jn.00994.2010
    URL: http://hdl.handle.net/2292/11463
    Other University of Auckland co-authors: Janusz Lipski
  • Cucchiaroni, M. L., Freestone, P. S., Berretta, N., Viscomi, M. T., Bisicchia, E., Okano, H., ... Mercuri, N. B. (2011). Properties of dopaminergic neurons in organotypic mesencephalic-striatal co-cultures - evidence for a facilitatory effect of dopamine on the glutamatergic input mediated by α-1 adrenergic receptors. European Journal of Neuroscience
    Other University of Auckland co-authors: Janusz Lipski
  • Freestone, P. S., Chung, K. K. H., Guatteo, E., Mercuri, N. B., Nicholson, L. F. B., & Lipski, J. (2009). Acute action of rotenone on nigral dopaminergic neurons--involvement of reactive oxygen species and disruption of Ca2+ homeostasis. Eur J Neurosci, 30 (10), 1849-1859. 10.1111/j.1460-9568.2009.06990.x
    URL: http://hdl.handle.net/2292/9223
    Other University of Auckland co-authors: Janusz Lipski

Contact details

Primary location

M&HS BUILDING 502 - Bldg 502
Level 4, Room 401
85 PARK RD
GRAFTON
AUCKLAND 1023
New Zealand