Dr Rebecca Helen Johnson

Research | Current

  • Drug discovery using primary human brain cells
  • Models of the diseased blood brain barrier using primary brain endothelial and diseased pericyte co-cultures, and vessels from post-mortem diseased brains. This research aims to determine the importance of pericytes in the maintenance of the BBB in Alzheimer's disease, Parkinson's disease and motor neuron disease.

Postgraduate supervision

Co-supervisor: Susan Li - PhD Pharmacology 

Distinctions/Honours

Neurologica Foundation First Fellowship (Barker Fellowship).

Areas of expertise

  • Blood brain barrier and neuroinflammation
  • Primary culture of brain endothelial cells and pericytes
  • Biosensor technology
  • Flow cytometry and cytometric bead array
  • Immunocytochemistry

Selected publications and creative works (Research Outputs)

As of 29 October 2020 there will be no automatic updating of 'selected publications and creative works' from Research Outputs. Please continue to keep your Research Outputs profile up to date.
  • Anchan, A., Kalogirou-Baldwin, P., Johnson, R., Kho, D. T., Joseph, W., Hucklesby, J., ... Graham, E. S. (2019). Real-Time Measurement of Melanoma Cell-Mediated Human Brain Endothelial Barrier Disruption Using Electric Cell-Substrate Impedance Sensing Technology. Biosensors, 9 (2).10.3390/bios9020056
    Other University of Auckland co-authors: Akshata Anchan, James Hucklesby, Graeme Finlay, Simon OCarroll, Scott Graham, Catherine Angel
  • Johnson, R. H. (2019). Systemic inflammatory regulation of blood-brain barrier inflammation The University of Auckland. ResearchSpace@Auckland.
    URL: http://hdl.handle.net/2292/49728
  • Gu, A. Y., Kho, D. T., Johnson, R. H., Graham, E. S., & O'Carroll SJ (2018). In Vitro Wounding Models Using the Electric Cell-Substrate Impedance Sensing (ECIS)-Zθ Technology. Biosensors, 8 (4).10.3390/bios8040090
    Other University of Auckland co-authors: Scott Graham, Simon OCarroll, Andrea Gu
  • Robilliard, L. D., Kho, D. T., Johnson, R. H., Anchan, A., O'Carroll SJ, & Graham, E. S. (2018). The Importance of Multifrequency Impedance Sensing of Endothelial Barrier Formation Using ECIS Technology for the Generation of a Strong and Durable Paracellular Barrier. Biosensors, 8 (3).10.3390/bios8030064
    Other University of Auckland co-authors: Simon OCarroll, Scott Graham, Laverne Robilliard, Akshata Anchan
  • Johnson, R. H., Kho, D. T., O' Carroll SJ, Angel, C. E., & Graham, E. S. (2018). The functional and inflammatory response of brain endothelial cells to Toll-Like Receptor agonists. Scientific reports, 8 (1)10.1038/s41598-018-28518-3
    Other University of Auckland co-authors: Simon OCarroll, Catherine Angel, Scott Graham
  • Kho, D. T., Johnson, R. H., O'Carroll SJ, Angel, C. E., & Graham, E. S. (2017). Biosensor technology reveals the disruption of the endothelial barrier function and the subsequent death of blood brain barrier endothelial cells to sodium azide and its gaseous products. Biosensors, 7 (4)10.3390/bios7040041
    Other University of Auckland co-authors: Catherine Angel, Simon OCarroll, Scott Graham
  • Kho, D. T., Johnson, R., Robilliard, L., du Mez, E., McIntosh, J., O'Carroll SJ, ... Graham, E. S. (2017). ECIS technology reveals that monocytes isolated by CD14+ᵛᵉ selection mediate greater loss of BBB integrity than untouched monocytes, which occurs to a greater extent with IL-1β activated endothelium in comparison to TNFα. PLoS One, 12 (7)10.1371/journal.pone.0180267
    Other University of Auckland co-authors: Catherine Angel, Julie McIntosh, Simon OCarroll, Scott Graham
  • O'Carroll SJ, Kho, D. T., Wiltshire, R., Nelson, V., Rotimi, O., Johnson, R., ... Graham, E. S. (2015). Pro-inflammatory TNFα and IL-1β differentially regulate the inflammatory phenotype of brain microvascular endothelial cells. Journal of Neuroinflammation, 1210.1186/s12974-015-0346-0
    Other University of Auckland co-authors: Scott Graham, Simon OCarroll, Catherine Angel, Lola Mugisho

Contact details

Primary office location

M&HS BUILDING 502 - Bldg 502
Level 5, Room 501
85 PARK RD
GRAFTON
AUCKLAND 1023
New Zealand